Figure 4.

The expression of dominant-negative RhoA (N19-RhoA) inhibits TGF-β–mediated EMT. Forty-eight hours after retroviral infection with N19-RhoA, dominant-negative JNK (JNK^APF), dominant-negative Rac1 (N17-Rac1), or GFP cDNA as a control, NMuMG cells were treated with TGF-β (5 ng/ml for 24 h at 37°C). Phase contrast and immunofluorescent E- and N-cadherin localization was observed. Expression of dominant-negative N17-RhoA blocked TGF-β induction of EMT, whereas expression of N17-Rac1 and JNK^APF did not.