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Rac1 and Cdc42 activation by TGF-β in NMuMG cells. TGF-β–treated (10 ng/ml for 0–60 min at 37°C) NMuMG cells were adsorbed to PBD-GST or run directly on a 12% acrylamide gel and immunoblotted sequentially for Rac1 and Cdc42. As a control, permeabilized cells incubated with GTPS were assayed for Rac1 activation (left). TGF-β–mediated induction of Rac1 and Cdc42 activation was negligible in NMuMG cells.
