Figure 7.

TGF-β regulation of p160^ROCK kinase activity is involved in the EMT of NMuMG cells. (A) Myc-tagged p160^ROCK was immunoprecipitated from cells treated with TGF-β (10 ng/ml for 0–360 min at 37°C) and phosphorylation of histone was resolved by electrophoreses followed by autoradiography. Expression of myc-tagged p160^ROCK was determined by Western blotting. TGF-β induces p160^ROCK kinase activity. (B) Cells retrovirally transduced with either GFP (as a control) or KD-IA p160^ROCK cDNA were treated with 5 ng/ml TGF-β for 18 h. Subsequently, cells were fixed and stained with for E-cadherin or F-actin. The panels (400×) are magnified as insets (800×). The data suggest p160^ROCK activity is involved in TGF-β–mediated actin stress fiber formation, but not E-cadherin delocalization.