Figure 8.

TGF-β–induced motility of NMuMG cells can be antagonized by blocking the PI3-kinase or RhoA signaling pathways. Retrovirally transduced NMuMG cells allowed to attach to 8 μM polycarbonate filters (Costar, Cambridge, MA) for 18 h, were transferred to wells with fresh media supplemented with 10% serum, with or without 5 ng/ml TGF-β and/or 10 nM LY294002 (LY). The bar graph represents colonies present after 48 h in the presence or absence of 5 ng/ml TGF-β in at least two experiments done in triplicate. Blocking PI3-kinase and RhoA signaling limited TGF-β–mediated motility of NMuMG cells.