Figure 1.

Interleukin-6 signals to the translational machinery through ERK and Mnk1. A, Western blot and quantification for p-ERK, total ERK, p-eIF4E, and total eIF4E following 15 min of treatment of DRG cultures with increasing doses of IL-6. B, Western blot and quantification for p-ERK, total ERK, p-eIF4E, and total eIF4E following 15 min of treatment of TG cultures with escalating doses of IL-6. C, Western blot and quantification for p-eIF4E and total eIF4E following pretreatment of DRG neurons in culture with the MEK inhibitor U0126 (10 μm) for 30 min and subsequent treatment with IL-6 (50 ng/ml) for 15 min. D, Western blot and quantification for p-eIF4E and total eIF4E following pretreatment of DRG cultures with the Mnk1 inhibitor CGP57380 (CGP, 50 μm) for 30 min and subsequent treatment with IL-6 (50 ng/ml) for 15 min. E, The Mnk1 inhibitor CGP57380 also inhibited IL-6-induced phosphorylation of eIF4E in TG neurons. F, IL-6 (50 ng/ml) did not stimulate mTOR phosphorylation in DRG neurons, and this was also not changed by CGP57380 (50 μm). Phosphorylated proteins were standardized relative to their respective totals and shown as percentage of vehicle. *p < 0.05, **p < 0.01.