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. Author manuscript; available in PMC: 2011 Mar 14.
Published in final edited form as: Science. 2010 Oct 15;330(6002):366–369. doi: 10.1126/science.1193573

Fig. 2.

Fig. 2

Effect of natriuretic peptides on gonadotopin-independent (spontaneous) resumption of meiosis and production of cAMP and cGMP in vitro (2A) Effect of natriuretic peptides on spontaneous maturation of cumulus cell-enclosed oocytes in vitro. Oocytes were cultured 4 hr in medium containing human NPPA, NPPB, or NPPC and assessed for germinal vesicle breakdown (GVBD) indicative of the resumption of meiosis. NPPC dose-dependently inhibited resumption of cumulus cell-enclosed oocytes with a maximum effect at 100 nM. Neither NPPA or NPPB had detectable activity in preventing GVBD at 100 nM. Effect of natriuretic peptides on levels of cGMP (2B) and cAMP (2C) in cumulus and oocyte compartments of cumulus-oocyte complexes (COCs). COCs were incubated in medium containing 100 nM human NPPA, NPPB or NPPC for 1 hr, and levels of cGMP and cAMP in oocytes and cumulus cells were evaluated by ELISA. NPPC, but not NPPA or NPPB, promoted increased cGMP levels in both oocytes and cumulus cells but an increase in cAMP was seen only in oocytes. CC indicates the total cumulus cells surrounding one oocyte. (*P < 0.05 compared with control).