Figure 2.

Lysine 93 is acetylated by p300 and lysines 95 and 67 are acetylated by p300 and PCAF. (A) HEK293 cells were co-transfected with PCAF and KLF8 or its KàR mutants as indicated. Lysates were subjected to immunoprecipitation using an anti-HA antibody and were blotted using an anti-acetyl-lysine or anti-HA antibody. The histogram depicts acetyla-tion levels relative to KLF8, which was normalized to 1. Results represent mean ± S.E. where n > 3. *, p<0.05. (B) Experiments were performed similarly as in A except that p300 was co-transfected as indicated. Expression of the transfected constructs was verified by whole cell lysate blotting (data not shown).