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. 2010 Nov 21;3(2):121–132.

Figure 5.

Figure 5

CtBP positively regulates KLF8 sumoyla-tion. (A) The KLF8 repression domain mutant cannot be sumoylated as efficiently as WT KLF8. HEK293 cells were transfected with HA-KLF8, HA-AVALF-KLF8 and Myc-SUMO-1 as indicated. Lysates were collected with NP-40 lysis buffer supplemented with 20 μM of the desumoylase inhibitor NEM followed by immunoprecipitation using an anti-HA antibody and western blotting (IB) using anti-Myc, anti-HA, and anti -CtBP antibodies. (B) Overexpression of CtBP promotes KLF8 sumoylation. HEK293 cells were transfected with HA-KLF8, Myc-SUMO, or His-CtBP as indicated. Experiments were performed as in (A). Shown are representative experiments where n=3. Expression of the transfected constructs was verified by whole cell lysate blotting (data not shown).