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. 2010 Nov 21;3(2):121–132.

Figure 7.

Figure 7

KLF8 acetylation at lysines 93 and 95 positively regulates cyclin D1 promoter activity. (A) PCAF or p300 can rescue CtBP mediated inhibition of KLF8 transcriptional activity. Luciferase activity was measured in T80 human ovarian epithelial cells that were co-transfected with the cyclin D1 promoter reporter and either the empty vector, KLF8, PCAF, p300, or CtBP in the indicated combinations. After 16 h, cells were harvested for luciferase assays as described in Materials and Methods. Results were normalized to vector control. *, p<0.05 compare column 5 with 2, or 6 and 7 with 5. (B) Acetylation at lysine 93 inhibits CtBP mediated inhibition of KLF8 transcriptional activity. Similar experiments were performed using WT-KLF8 or the KLF8-K93R and K93Q mutants. *, p<0.01 compare column 3 with 2. **, p<0.01 compare column 6 with 2. ***, p<0.05 compare column 12 with 6. (C) Acetylation at lysine 93 inhibits CtBP mediated inhibition of KLF8 transcriptional activity. Similar experiments were performed using WT-KLF8 or the KLF8-K95R and K95Q mutants. *, p<0.01 compare column 3 with 2. **, p<0.01 compare column 6 with 2. ***, p<0.01 compare column 12 with 6. All experiments were done in triplicate and the results represent mean ± S.E. where n>3.