Figure 4.

Validation of differentially expressed proteins by immunoblotting. (A) Representative im-munoblots for rodent plasma proteins. For the assessment of aldo A and apoE, plasma samples were resolved on an SDS-PAGE gel before immunoblotting. For VDBP, a native PAGE gel was run to preserve protein integrity of VDBP isoforms. Equal quantities of protein were loaded and confirmed by fibronectin. (B) Immunoblot data was quantified as a ratio of injury/control at each timepoint. * indicates statistically significant from control sample at the same time point (P<0.05). The horizontal line depicts the relative level of the control sample.