(A) Quantitative real-time PCR analysis of the expression of Bfl-1, GADD45β, and cIAP1 in wild-type mouse primary hepatocytes that were pretreated with vehicle (DMSO), GW4064 (2 μM) or 6ECDCA (3 μM) for 18 h before treatment with TNF-α (10 ng/ml) for 6 h. (B, C) Quantitative real-time PCR analysis of the expression of Traf1, Traf2, cIAP2 and Bfl-1 in livers from wild-type (B) or FXR−/− (C) mice that were transfected with ConAd or FXRAd (n=4). The mice received a single dose of LPS (30 mg/kg) or PBS as control.