Skip to main content
. Author manuscript; available in PMC: 2011 Mar 14.
Published in final edited form as: Hepatology. 2008 Nov;48(5):1632–1643. doi: 10.1002/hep.22519

Figure 8. Activation of NF-κB by TPA, LPS and p65 overexpression antagonizes FXR transactivation.

Figure 8

(A, B) Relative luciferase activities of HepG2 cells that were co-transfected with the FXR reporter plasmid EcRE-LUC, the control plasmid phRL-TK, and/or FXR/RXR expression plasmids, and pre-treated with GW4064 or vehicle (DMSO) for 18 h before treatment with TPA (50 nM) (A) or LPS (1 μg/mL) (B) for 6 hours. *P < .05, **P < .005 (n=3); (C) Relative luciferase activities of HepG2 cells were co-transfected with the FXR reporter plasmid EcRE-LUC, the control plasmid phRL-TK, and increasing amounts of a p65 expression plasmid at 0.5:1, 1:1 or 3:1 ratios with FXR/RXR expression plasmids, and then treated with GW4064 or vehicle (DMSO) for 24 h. *P < .05 compared to the control group; #P < .05 (n=3).