TABLE 1.
Body and liver weights, liver MDA, plasma transaminases, mitochondrial Prx-SO3, and TNFα concentrations in WT and Ppara-null mice fed STD or HFD for 3 wk1
| Measurement | WT-STD | WT-HFD | Null-STD | Null-HFD | Two-way ANOVA2 |
| Liver, weight, g/100 g body | 2.87 ± 0.07 | 3.06 ± 0.08 | 4.06 ± 0.06 | 4.55 ± 0.23 | G: P < 0.05 |
| ALT, U/L | 36.3 ± 8.95 | 43.3 ± 6.39 | 60.7 ± 9.13 | 38.5 ± 10.0 | |
| AST, U/L | 147 ± 7.00 | 158 ± 11.9 | 189.8 ± 25.8 | 201 ± 22.9 | |
| Cytosolic MDA,3μmol/L | 4.67 ± 0.07c | 6.65 ± 0.56b | 6.56 ± 0.39b,c | 10.9 ± 0.48a | G, D: P < 0.0001; GxD: P < 0.05 |
| Mito. Prx-SO3, % WT-STD | 100 ± 0.57b | 98.1 ± 3.82b | 102 ± 6.99b | 399 ± 19.4a | G, D, GxD: P < 0.0001 |
| TNFα, pg/50 μg protein | 46.8 ± 8.20 | 104 ± 12.4 | 67.3 ± 8.93 | 164 ± 8.20 | G: P < 0.005 D: P < 0.0001 |
1
Data are means ± SEM, = 4 or 3 (WT-STD). Means in a row with superscripts without a common letter differ, P < 0.05.
2
G, genotype; D, diet.
3
Cytosolic proteins (20 g/assay) were used to measure MDA levels in different groups.