TABLE 2.

Trabecular measurements in tibia of young, middle-aged, and old rats fed diets differing in VA concentration for 2–3, 8–10 or 18–20 mo¹²

Age	Diet	n	Trabeculae, 2	Trabeculae thickness, nm	Trabecular BMD,3mg mineral/mm3
Young	Mar	6	3.7 ± 0.02a	74 ± 2c	657 ± 7d
	Con	9	3.4 ± 0.11b	71 ± 3c	666 ± 7d
	Sup	6	3.6 ± 0.25a,b	69 ± 4c	664 ± 10d
Middle	Mar	5	2.1 ± 0.05c	147 ± 1a	838 ± 4b
	Con	6	2.4 ± 0.13c	129 ± 2b	807 ± 4c
	Sup	7	2.5 ± 0.12c	130 ± 4b	813 ± 8c
Old	Mar	8	1.3 ± 0.05e	144 ± 5a	887 ± 5a
	Con	7	1.4 ± 0.07d,e	119 ± 7b	845 ± 10b
	Sup	6	1.8 ± 0.20c,d	131 ± 6b	849 ± 5b
Two-way   ANOVA P-values
Diet			0.043	0.0001	0.0007
Age			0.0001	0.0001	0.0001
Interaction			0.032	0.05	0.004

¹Values are means ± SEM. Means in a column without a common letter differ, P < 0.05.

²Mar, marginal; Con, control; Sup, supplemented diet.

³Illustrations from representative samples can be found in Supplemental Figure 4.