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. 2010 Dec 1;117(5):1530–1539. doi: 10.1182/blood-2010-06-293167

Figure 4.

Figure 4

Reduced erythroid and B lymphocytes in the bone marrow, spleens, and peripheral blood of mice receiving Hspa9-shRNA–transduced progenitor cells. (A) The percentage of donor (Ly5.1+)–transduced (YFP+) cells was measured in the bone marrow, spleens, and peripheral blood of recipient mice up to 8 weeks after transplantation (n = 8-9). (B) Mice were given 2 intraperitoneal injections of BrdU 12 and 24 hours prior to isolating YFP+ bone marrow cells and staining them for BrdU incorporation as a measure of cells in the S phase of the cell cycle. (n = 4). (C-D) The contribution of YFP+ erythroid precursors was measured in the bone marrow, spleens, and peripheral blood of recipient mice up to 8 weeks after transplantation (n = 8-9). (E-G) The contribution of YFP+ B cells, T cells, neutrophils, and monocytes was measured in the bone marrow, spleens, and peripheral blood of recipient mice using flow cytometry up to 8 weeks after transplantation (n = 8-9). All data represent the means ± SD.