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. 2010 Dec 1;117(5):1555–1564. doi: 10.1182/blood-2010-06-288621

Figure 2.

Figure 2

EmTh-1 supernatant impairs TGF-β–induced iTreg generation and promotes Tbet+ T-lymphocyte differentiation. CD4+CD25−CD62L+ naive T cells were cultured for 72 hours with T-cell expander beads (cell:bead ratio 1:1) with or without TGF-β1 (5 ng/mL) in the presence or absence of the emTh-1 supernatant (emTh-1 sup). Cells were then analyzed by flow cytometry. (A-B) Representative dot plots or histogram plots from 10 independent experiments. (C) Percentage of CD4+CD25+FoxP3−- activated T cells in total CD4+ T lymphocytes. *P < .01, a significant difference compared with cells cultured without emTh-1 supernatant. (D) The expression of the transcription factors FoxP3, Tbet, and GATA-3 was determined in CD4+CD25−CD62L+ T cells cultured for 72 hours with T-cell expander beads with or without TGF-β1 treated or not with emTh-1 supernatant. Results are representative of 3 independent experiments.