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. 2010 Dec 1;117(5):1555–1564. doi: 10.1182/blood-2010-06-288621

Figure 3.

Figure 3

EmTh-1 cell–mediated inhibition of TGF-β–induced iTreg generation depends on IFN-γ. CD4+CD25−CD62L+ naive T cells were cultured for 72 hours with T-cell expander beads with or without TGF-β1 in the presence or absence of emTh-1 supernatant and with or without blocking antibodies against (A) mouse IFN-γ (*P < .05, a significant difference compared with the TGF-β + emTh-1 group) or (B) mouse TNF-α. (C-D) CD4+CD25−CD62L+ naive T lymphocytes were isolated from mouse spleens (IFN-γR−/−) and cultured for 72 hours with T-cell expander beads with or without TGF-β1 and in the presence or absence of emTh-1 supernatant. Percentage of FoxP3-expressing cells was determined by flow cytometry. Results of 3 independent experiments have been combined. *P < .01, a significant difference compared with cells from wild-type (IFN-γR+/+) mice cultured in the same conditions.