Table 1.

List of primers used for qRT-PCR analysis

Gene	Forward primer	Reverse primer
GAPDH (82F/182R)	5′-AGGGCTGCCTTCTCTTGTGAC-3′	5′-TGGGTAGAATCATACTGGAACATGTAG-3′
β-actin (221F/322R)	5′-TGGGTCAGAAGGACTCCTACGT-3′	5′-CGTCCCAGTTGGTGACAATG-5′
hepcidin (165F/252R)	5′-TGACAGTGCGCTGCTGATG-3′	5′-GGAATTCTTACAGCATTTACAGCAGA-3′
BMP2 (201F/311R)	5′-ACGTCCTCAGCGAGTTTGAGTT-3′	5′-GGCGGTACAGGTCGAGCATA-3′
BMP4 (1534F/1644R)	5′-GAGCGCCATTTCCATGTTGT-3′	5′-CTCCGGACTGCCTGATTTCA-3′
BMP5 (807F/938R)	5′-CCACAGAACAATTTGGGCTTACA-3′	5′-CTTGAAGAAGGCCACCATGAA-3′
BMP6 (871F/971R)	5′-GTGTGGGCCTCCGAAGAA-3′	5′-ACACTCAGCTGGAGTCCCATGT-3′
BMP9 (741F/831R)	5′-CCAAAACCTGCCCTTCTTTGT-3′	5′-GTGGCCAATCATCTCCTTCAG-3′
TMPRSS6 (2364F/2464R)	5′-AGAAGGTGGATGTGCAACTGATC-3′	5′-CTTGCCCTTGCGATAACCA-3′
TGF-β1 (1238F/1338R)	5′-CACCGGAGAGCCCTGGATA-3′	5′-TTCCAACCCAGGTCCTTCCT-3′
Tf receptor 1(2227F/2302R)	5′-AGTGGTCGCTGGGTGTGATT-3′	5′-CCTTCAGGCATACAGCTCAATTG-3′
HAI-1 (1164F/1264R)	5′-CCCATTCAGTGAACGCTGTG-3′	5′-TGCCACGACAGGACTCAAGA-3′
HAI-2 (437F/537R)	5′-TCCCCAGGTGGTGGTACAAT-3′	5′-ACACTCCTCCTTGCTTGGATAGTT-3′

BMP indicates bone morphogenic protein; GAPDH, glyceraldehyde 3-phosphate dehydrogenase; HAI, hepatocyte growth factor activator inhibitor; qRT-PCR indicates quantitative real-time reverse transcription polymerase chain reaction; Tf, transferrin; and TGF, transforming growth factor.