Fig. 2.

The Caprin-1 5′ flanking region is regulated by Pou4f3. (A) Schematic showing the luciferase reporter constructs containing the Caprin-1 5′ flanking sequence used in the co-transfection experiments shown in B. Each construct was co-transfected into OC-2 cells with either a Pou4f3 or mutant Pou4f3 Driedel (ddl) expression vector. (B) Dual luciferase assays were used to determine promoter activity, which is expressed as a percentage of promoter induction relative to activity with the empty expression vector. ug, μg of expression vector. Error bars represent s.e.m, (n=6). Student's t-tests were performed comparing each condition with 0 μg of Pou4f3 expression vector. *P<0.05; #P<0.005; ##P<0.001.