Fig. 5.

Changes in TIA-1 and Caprin-1 expression during neomycin treatment. Cochlear explant cultures from P2 rats were treated with 1 mM neomycin for 0, 3, 6 or 12 hours. Caprin-1, TIA-1 and myosin VIIa expression were determined by triple-label immunofluorescence, with DAPI to assess chromatin structure. Areas shown are from the basal end of cochlear coils and were selected without observing fluorescent signals, to avoid bias. Images are the averages of four confocal optical sections representing ~6-μm-optical-slices of the outer hair cell region. The upper and lower rows represent focal planes of the upper apical pole and lower nuclear pole of hair cells, respectively (see inset). Arrowheads indicate hair cell granules labelled with both Caprin-1 and TIA-1 (also see supplementary material Fig. S2). Asterisks indicate condensed nuclei. Scale bar: 10 μm (for all images).