Fig. 1.
Single-labelling of atrial myocytes. An atrial myocyte segment labelled with antibodies against RyR2 (A), Casq (B), Cav1.2 (C), Ncx (D) or Cav3 (E). For each antibody, the image shown is either a 1.5-μm-thick section taken from the cell surface (left panels), or a single 250-nm plane taken from approximately the middle of the cell (right panels). For the interior segments of RyR2 (A) and Casq (B), there is a discrete subsarcolemmal ‘gap’ with no labelling of either protein (upper inset, arrows), whereas the lower inset shows that some longitudinal elements of labelling are present (arrowheads). Scale bar: 5 μm.