Fig. 3.
Triple-labelling of RyR2, Ncx and Casq. An atrial myocyte segment labelled with antibodies against RyR2 (blue), Ncx (green) and Casq (red). All images are 1-μm-thick slices from the cell surface. (A) View of the entire cell, showing surface distribution and colocalization of RyR2, Ncx and Casq. (B) Segment of the cell surface from the area shown in A. (C) The same segment, showing only the object colocalization of RyR2–Ncx–Casq (white), RyR2–Casq (magenta), RyR2–Ncx (cyan) and Ncx–Casq (yellow). Scale bars: 5 μm.