Fig. 4.
Triple-labelling of RyR2, Cav3 and Cav1.2. An atrial myocyte labelled with antibodies against RyR2 (blue), Cav3 (green) and Cav1.2 (red). All images are 1-μm-thick slices from the cell surface. (A) View of the entire cell, showing surface distribution and colocalization of RyR2, Cav3 and Cav1.2. (B) Segment of the cell surface from the area shown in A. (C) The same segment, showing only the object colocalization of RyR2–Cav3–Cav1.2 (white), RyR2–Cav1.2 (magenta), Cav3–Cav1.2 (yellow) and RyR2–Cav3 (cyan). Scale bars: 5 μm.