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. 2011 Mar 14;6(3):e14757. doi: 10.1371/journal.pone.0014757

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Hashemi et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Methylation at CCGG sites measured by LUMA in PPP sensitive Line2 and tolerantLine2-T500 cells. The Y-axis shows the HpaII/MspI ratio, which correlates inversely to DNA methylation levels. Line2 cells cultured in PPP free media were short-term treated for 24 h with or without 500 nM PPP. Line2-T500 cells cultured in 500 nM PPP were similarly treated for 24 h by adding 500 nM PPP (total concentration 1000 nM). Peak heights were plotted with error bars ± SD. P-values were calculated using the t-test, n.s. = not statistically significant.