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. 2011 Mar 14;6(3):e17519. doi: 10.1371/journal.pone.0017519

Figure 4. Fibrinogenolytic activity of eupolytin1 and its effects by metal ions and protease inhibitors.

Figure 4

A: native and recombinant eupolytin1 (1 µg) were incubated with 20 µg fibrinogen for 1 h at 37°C and subjected reduced SDS-PAGE analysis in a gel concentration of 15%; B: 1 µg eupolytin1 was incubated with 20 µg fibrinogen for 1 h at 37°C in the presence of 5 mM indicated metal ion and subjected reduced SDS-PAGE analysis in a gel concentration of 15%; C & D: 1 µg eupolytin1 was incubated with 20 µg fibrinogen for 1 h at 37°C in the presence of PMSF (C) or aprotinin (D) with indicated concentration and subjected reduced SDS-PAGE analysis in a gel concentration of 15%. NE: native eupolytin1; RE: recombinant eupolytin1.