Table 1. Kinetic parameters of eupolytin1 for different chromogenic substrates.
T6140 | B3133 | |||
NE | RE | NE | RE | |
Km (mM) | 20.17 | 20.02 | 3.24 | 3.01 |
Vmax (mM.S−1) | 2.47 | 2.39 | 1.05 | 1.12 |
Kcat (105 S−1) | 2.10 | 2.25 | 0.32 | 0.29 |
Kcat/Km (105 mM−1 S−1) | 0.10 | 0.11 | 0.10 | 0.08 |
These data represent mean values of three independent experiments. NE: native eupolytin1; RE: Recombinant eupolytin1.