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. 2011 Mar 14;6(3):e17831. doi: 10.1371/journal.pone.0017831

Figure 2. Binding of monomeric and trimeric p17 to p17R and induction of MCP-1 production.

Figure 2

(A, B, C) Biotin-conjugated monomeric and trimeric p17 at 50 (black line), 200 (grey line) and 400 ng/ml (dotted line) was allowed to react with Raji (A), primary B cells (B) and H9 (C). Cells incubated with biotinylated GST were used as negative control (solid histogram). Binding of p17 to cells was detected by using APC-conjugated streptavidin. Data were analyzed using CELLQUEST Software and displayed as histograms. These results are representative of four different experiments with similar results. (D) Purified human monocytes were treated or not with GST, monomeric and trimeric p17 at a concentration of 1 µg/ml. Culture supernatants were collected 48 h after the stimulation of culture and analysed for the presence of MCP-1 by a standard quantitative ELISA. Bars represent the mean ± SD of four independent experiments performed in triplicate. Statistical analysis was performed by Wilcoxon matched pairs test. *** P<0.001, statistically different compared with GST.