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. 2011 Mar 14;6(3):e17831. doi: 10.1371/journal.pone.0017831

Figure 4. AP-1 activation in p17- and p17Δ36-treated Raji cells.

Figure 4

(A, B) Nuclear extracts obtained from Raji cells collected after p17 and p17Δ36 treatments (1 µg/ml) were analysed for their binding activity to oligonucleotides specific for the transcription factor AP-1 and Oct-1. (A) Representative EMSA autoradiograms using nuclear extracts (10 µg per sample) from Raji cells collected at specific times 0.5, 1, 2 and 4 h after p17 and p17Δ36 treatment, and AP-1-specific radiolabeled oligonucleotides are shown. NS: p17-untreated cells. Protein-DNA complex specificity was confirmed by competition with an excess of unlabelled oligonucleotide probes. DNA-binding activity to Oct-1 was used as loading control. (B) The panel represents the densitometric analysis of changes in DNA-binding activity of AP-1 relative to Oct-1, expressed as fold of induction over p17-untreated cells. Ratios were expressed on a logarithmic scale in base 2. Bars represent the mean ± SD of four independent experiments. Statistical analysis was performed by two-way ANOVA. Bonferroni's post test was used to compare data: *** P<0.001.