Figure 1.

Detection of viral RNA by RT-PCR in brains of mice inoculated with Japanese encephalitis virus. The size of the amplified fragment was estimated to be 291 bp. Viral RNA was observed in all mice with evident clinical symptoms (m1, m2, and m4). A mouse (c1) inoculated with culture medium was used as the control. In addition, viral RNA was detected from both the cerebrum (marked with B) and the cerebellum (marked with b) of brains of infected mice. The lane marked "v" is the positive control taken from a cultured virus suspension.