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. 2011 Mar 3;10:15. doi: 10.1186/1475-2859-10-15

Figure 1.

Figure 1

Expression analysis of the synthetic gene variants by SDS-PAGE. Lane 1, molecular weight marker. Lane 2, lysate of E. coli W3100 culture harboring the pWT expression vector for the expression of wild type calf prochymosin gene grown in the absence of L-arabinose; lanes 3-10, lysate of E. coli W3100 culture harboring the pV0, pV1, pV2, pV3, pV4, pV5 and pV6 expression vector for the expression of V0-V6 synthetic versions of calf prochymosin grown in the with 2 g/l of L-arabinose. In all cases, cell cultures were brought to OD600 = 3 and 20 μl were used for the analysis.