Table 1.
Primers used in this study.
| Primer | Sequence | Target |
|---|---|---|
| NP-F1(+) | ATACGTGGCAGTCACAACCT | NP1 |
| NP-R1(-) | CGTCTGTTAC CTCCTCTGAT | NP1 |
| NP-F2(+) | ATGAGCTCCGAATCTATG | NP1* |
| NP-R2(-) | CCTCTGATTCCTGTGAAG | NP1 |
| VP-F1(+) | AACGACTGGTCTCTTGGTGGCAT | VP1 |
| VP-R1(-) | TGGATATGCG TGTTCACCAT CAC | VP1 |
| VP-F2(+) | CGACTGGTCTSTTGGTGGCATTAT | VP1# |
| VP-R2(-) | CGTTGTTGTA TGTAGTGTCA GCACC | VP1 |
* The target fragment of NP1 partial gene was 586 nt, in 2285-2870 nt according to HBoV2 FJ170279 strain.
# The target fragment of VP1 partial gene was 620 nt, in 3134-3753 nt according to HBoV2 FJ170279 strain.
‡The target fragment of NP1 Real-time PCR 145 nt, in 2489-2633 nt according to HBoV2 FJ170279 strain.