Table 7.

RT-PCR confirmation of differences in gene expression: Growth/neurotrophic factor genes from Experiment 2.

		ALC-NTC				ALC-NTO
		Microarray		qRT-PCR		Microarray		qRT-PCR
Gene symbol	GenBank	Fold Change*	p-value*	Fold Change*	p-value*	Fold	p-value*	Fold Change*	p-value*
Ctgf	NM_010217	-1.7	0.01	-1.2	0.300	-2.2	0.004	-2.0	0.048
Edil3	NM146015	-1.6	0.00	-1.4	0.019	-1.9	0.025	Not tested
Efemp1	NM_146015	-1.5	0.01	-2.3	0.002	-2.3	0.001	-1.8	0.010
Igf1	NM_184052	-1.4	0.02	-2.6	0.001	-1.3	0.060	Not tested
Igfbp2	NM_008342	-2.0	0.02	-1.6	0.084	-1.5	0.035	-2.0	0.045
Ntf3	NM_008742	-1.2	0.30	-1.7	0.060	-1.8	0.030	-1.9	0.038
Tieg1	NM_013692	-1.2	0.36	-1.3	0.015	-1.5	0.010	-1.2	0.053

*Control versus alcohol-treated

Fold change: positive = ratio of alcohol-treated to control, negative = ratio of control to alcohol-treated. p-value: t-test of control vs. alcohol treated