Figure 1.

Effects of CCCP and CGP37157 on Ca²⁺ release from mitochondria after high-K⁺ cell stimulation. Chromaffin cells expressing mitochondrial mutated aequorin reconstituted with coelenterazine n were stimulated by 10-s pulses of medium containing 70 mM K⁺ (indicated at the bottom). When indicated, cells were perfused with 2 μM CCCP added immediately after the high-K⁺ pulse (CCCP), with 10 μM CGP37157 (CGP, preincubated for 2 min before high-K⁺ stimulation), or with both together. For [Ca²⁺]_M calculations, total luminescence was reduced 50%, assuming that [Ca²⁺]_M changes take place in only 50% of the mitochondrial space.