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Ca2+-induced Ca2+ release from depolarized Ca2+-loaded mitochondria. Cells expressing mutated aequorin reconstituted with coelenterazine n were permeabilized on line as described in MATERIALS AND METHODS. The arrows indicate 3-s pulses with intracellular medium containing a 10 μM [Ca2+] buffer. When indicated, either 2 μM CCCP, 10 μM CGP37157 (CGP), or 4 μM ruthenium red (RR) was perfused.
