Table 3.
Quantitative detemination of virus titers
| Virus | EM particles/ml | PCR genomes/ml | Infectivity FFU/ml | log10 difference PCR to EM | log10 difference FFU to EM | log10 difference FFU to PCR |
|---|---|---|---|---|---|---|
| EBOZV Mayinga | 2.5 × 108 | 8.3 × 1010 ± 3.2 × 109 | 1.0 × 105 | +2 | -3 | -5 |
| EBOSV Maridi | 7.5 × 108 | 8.6 × 1010 ± 5.4 × 109 | 1.0 × 103 | +2 | -5 | -7 |
| EBOSV Boniface | 8.0 × 108 | 5.9 × 1010 ± 3.4 × 109 | 1.0 × 104 | +2 | -4 | -6 |
| MARV Ravn | 4.0 × 108 | 1.3 × 108 ± 1.8 × 106 | 1.0 × 105 | 0 | -3 | -3 |
| MARV Ozolin | 5.0 × 109 | 1.6 × 109 ± 1,1 × 108 | 1.0 × 106 | 0 | -3 | -3 |
| MARV Musoke | 3.0 × 109 | 5.8 × 108 ± 2.7 × 107 | 1.0 × 105 | -1 | -4 | -3 |
| LASV Josiah | 1.0 × 106 | 2.2 × 107 ± 4.7 × 106 | 1.0 × 104 | +1 | -2 | -3 |
| RVFV ZH548 | 2.5 × 109 | 2.9 × 1011 ± 4.3 × 109 | 1.0 × 106 | +2 | -3 | -5 |
| DENV-1 231 | 3.0 × 109 | 4.6 × 1010 ± 4.3 × 109 | 1.0 × 106 | +1 | -3 | -4 |
| YFV Asibi | 4.0 × 108 | 8.2 × 109 ± 1.7 × 108 | 1.0 × 108 | +1 | 0 | -1 |
| CCHFV 10200 | 2.5 × 107 | 1.0 × 106 ± 6.1 × 105 | 1.0 × 106 | +1 | -1 | -2 |
EM: calculated from 10 grid squares, qPCR: average and standard deviation of triplicate qPCR results (8 qPCR results for CCHFV), FFU: mean of three titrations.