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A single nucleotide difference leads to the existence of an addition BamHI site of CDV field strains. All CDV isolates have an A at 610 nt in N gene 829 bp fragments, but vaccine strains have a G. (BamHI site GGATCC showed in box). Underline indicates the 19 Chinese CDV field strains analyzed in this study. Underline with broken line means commercial CDV vaccines analyzed in this study.
