Fig. 7. In vitro membrane binding of V12 Rac. Cytosol from attached or suspended V12 Rac cells was incubated with equal amounts of membranes from attached or suspended NIH 3T3 cells. Membranes were collected by centrifugation and V12 Rac detected by Western blotting with anti-Myc. Western blotting for the integrin β1 subunit demonstrates equivalent levels of membrane protein in each sample. Data are representative of three independent experiments.