Fig. 10. Phenotype of transformed colonies obtained from chicken hematopoietic progenitor cells infected with ts21-DNMafB, ts21 or ts21-MafB viruses. (A) FACS profiles of cells from pooled colonies of methylcellulose cultures using monoclonal antibodies against the multipotent progenitor antigen MEP21 or the myeloid antigen Myl51/2. The dotted line overlay shows the staining with an isotype-matched control monoclonal antibody against an intracellular antigen. (B) Graph showing the ratio of Myl51/2-positive to MEP21-positive cells calculated from the average of three colony assays each from ts21-DNMafB-, ts21- and ts21-MafB-infected cells.