Fig. 4. Immunofluorescence labelling of L.mexicana and L.donovani LPG-deficient mutants using anti-phosphoglycan mAbs. (A–D) mAb LT6. (A) L.mexicana wild type; (B) L.mexicana Δlmexlpg1, clone I/8D at the same exposure time as in (A). (C) L.mexicana Δlmexlpg1, clone I/8D exposed 10 times longer than in (A). Flagellar pockets are indicated by arrows. (D) L.mexicana Δlmexlpg1, clone I/8D + pXlmexlpg1 exposed for half the time in (A). (E and F) mAb L7.25. (E) L.mexicana wild type; (F) L.mexicana Δlmexlpg1, clone I/8D at the same exposure time as in (E). (G and H) mAb LT17. (G) L.mexicana wild type; (H) L.mexicana Δlmexlpg1, clone I/8D at the same exposure time as in (G). (I and J) mAb LT6. (I) L.donovani wild type; (J) L.donovani R2D2 at 10 times the exposure time in (I). (K and L) mAb L7.25. (K) L.donovani wild type; (L) L.donovani R2D2 at the same exposure time as in (K).