Fig. 5. FACS analysis of live L.mexicana wild-type (WT), LPG-deficient mutant (LPG^–) and episomal lmexlpg1 addback (LPG^– + pXlmexlpg1) promastigotes. (A) mAb L3.8; (B) mAb LT6; (C) mAb L7.25; (D) mAb LT17; (E) mAb LT6; (F) mAb LT17. The fluorescence signal of the three LPG-deficient mutants overlaps completely in (A), (B) and (D), and partially in (C). The control lacking the primary antibody (–mAb control, shaded grey) is only shown in (E) and (F), but was identical in (A–D).