Fig. 6. Analysis of L.mexicana SAP phosphoglycosylation, macrophage binding and macrophage infection by L.mexicana wild-type and LPG-deficient mutant (LPG^–) promastigotes. (A) ELISA of LT8.2-bound SAP released from L.mexicana wild-type (bar 1) and L.mexicana Δlmexlpg1, clone I/8D (bar 2) using the anti-phosphoglycan cap mAb L7.25 and the anti-repeat mAb WIC108.3. (B) Binding of L.mexicana wild-type and an LPG-deficient L.mexicana mutant (clone II/5C) to peritoneal macrophages. The bars represent the average of three experiments. The standard error is indicated. (C and D) Infection of peritoneal macrophages by L.mexicana wild type and LPG-deficient L.mexicana mutants (clones I/8D and II/5C). The ratio of infected to uninfected macrophages 6 days after a challenge with two promastigotes/cell is shown in (C), while (D) shows the percentage of infected macrophages with an amastigote burden >10 after a challenge with two promastigotes/cell of L.mexicana wild-type or LPG-deficient L.mexicana mutants (clone I/8D and clone II/5C) after 7 days in culture. The bars represent the average of duplicate determinations and the standard error is indicated. A representative of three separate experiments with similar results is shown. (E) Time course of infection of peritoneal macrophages after a challenge with two promastigotes/cell of L.mexicana wild-type or an LPG-deficient L.mexicana mutant (clone I/8D). (F) Time course of parasite burden after infection of peritoneal macrophages with two promastigotes/cell of an LPG-deficient L.mexicana mutant (clone I/8D). (E and F) Each time point was determined in duplicate experiments. The standard error is indicated. Identical experiments with clone II/5C gave the same results.