Fig. 7. Immunofluorescence of saponin-permeabilized peritoneal macrophages infected with L.mexicana wild-type (WT) and LPG-deficient mutant (LPG^–) promastigotes. Peritoneal macrophages were infected with five promastigotes of L.mexicana wild-type (A, C and E) or of L.mexicana Δlmexlpg1, clone I/8D per cell (B, D and F). Infected macrophages were labelled after 2 days in culture with the mAbs LT6 (A and B), L7.25 (C and D) and LT17 (E and F) (orange fluorescence). Macrophages and Leishmania nuclei and kinetoplasts were stained with DAPI (blue fluorescence). Uninfected macrophages showing only background fluorescence are indicated by arrows.