Figure 2.

Cell cycle phenotypes of wild-type and kinase target site deficient mutants of Op18. K562 cells were transfected, and hygromycin resistant cell lines were selected as in Figure 1. (A) Op18 expression was thereafter induced with Cd²⁺ (0.05 μM) for 24 h (upper panels) or 72 h (lower panels). DNA was stained with propidium iodide and analyzed by flow cytometry. The insert in the upper right panel shows the effect of nocodazole (0.5 μM) on Op18-tetraE expressing cells. Data are representative for three independent experiments.