Figure 7.

Cell cycle phenotypes of nonphosphorylatable Ala-substituted Op18 deletion derivatives suggest that catastrophe-promotion is essential for a mitotic block. K562 cells were transfected and hygromycin resistant cell lines were selected as in Figure 1. (A) Op18 expression was induced with Cd²⁺ for 24 h (upper panels) or 72 h (lower panels). DNA was stained with propidium iodide and analyzed by flow cytometry. The inserts in two of the upper panels show G2/M block after 24 h in presence of nocodazole (0.5 μM). Data are representative for two independent experiments.