Analysis of non-clathrin coat protein zeta 1-COP gene expression. Non-clathrin coat protein zeta 1-COP gene is represented by two different ESTs in SPODOBASE. A) SF9L03957-Contig1 (EST1) and Sf1P09405-5-1-Contig1 (EST2) that exhibit divergent expression patterns on microarrays. The fold change in expression for each probe, designated p1 - p6, at each time point is the average from four independent biological replicates. B) Diagram shows the relationship between EST1 and 2 and the locations of the microarray probes. Sequence comparisons reveal that both contigs share two common regions of nearly identical nucleotide sequence, indicated by the shaded black bars. The remaining sequences are unique to each EST, and depicted by either unfilled white or shaded grey bars. Probe 1 (p1) matches both contig sequences, p2-p3 are specific for the unique region of EST1, and p4 and p6 are specific for the unique region of EST2. Most of p5 is specific for the unique region of EST2, however it overlaps the small region of sequence identity shared with EST 1 (13 identical nucleotides).