Fig. 3.

Detailed description of the endogenous retroviral insertion site in the Nox3^het allele. a Schematic describing the endogenous retroviral (ERV) insertion in the Nox3^het allele. Central horizontal line, intron 12 of the Nox3 gene; green rectangle, ERV; red rectangles, target site duplications (TSD); black sequences, partial sequence from intron 12; red sequences, target site duplication; green sequences, partial ERV sequences showing similarity to ERV classes RLTR13B and RLTR13A2; nucleotide (nt) numbers, Chromosome 17 genome positions (build 37); black flags, PCR primers; gray lines, amplification products from the wild-type and mutant alleles. b (inset) The Nox3^het genotyping assay detects a 326-bp fragment in wild-type mice, a 241-bp fragment in Nox3^het mutant mice, and both fragments in heterozygous mice