Table 1.
Oligonucleotides
| Nox3het | |
| Genotyping | |
| Nox3-int12F | GTTCTGGAGCACCACCTTGT (use at 200 nM) |
| Nox3-int12R | CCCATAGGGAGCCAAGAAAT (use at 300 nM) |
| ERV-R | TGTCAAGCTGACTCCACCAG (use at 100 nM) |
| RACE | |
| 3′RACE outer primer | GCGAGCACAGAATTAATACGACT |
| 3′RACE inner primer | CGCGGATCCGAATTAATACGACTCACTATAGG |
| 3′NoxInH | CGCAAGCTTTATTTCACTACCCCGTGAGC |
| 3′NoxOut | CATGCCGAGGCTAGCAGT |
| 3′RaceInX (3′ RACE adapter) | CGCCTCGAGGAATTAAATACGACTCACTATAGGT12VN |
| Nox3het–2J | |
| Genotyping | |
| Nox3-10F | GGCAGAGGAGAGATTTGAGG |
| Nox3-10R | TGGGATGTCTTTGTGTCCAA |
| Nox3het–3J | |
| Genotyping | |
| Nox3-673F | TGACAACTTCTGCATTTCATCTG |
| Nox3-939R | GGAGGATGATGAAAACATTGAAG |
| RT-PCR | |
| Nox3ex-1F | TGTCATGCCGGTGTGCTGGA |
| Nox3ex-2R | AGGCGTTTACTGCCAGCCAT |
| Nox3ex-4R | CCCGTAGGCAACGAGTTTGTGGA |
| Nox3het–4J | |
| Genotyping | |
| Nox3-10F | GGCAGAGGAGAGATTTGAGG |
| Nox3-10R | TGGGATGTCTTTGTGTCCAA |
| RT-PCR | |
| Ap3d1ex-1F | TCCGCAACCACAAGGAGGACGA |
| Ap3d1ex-8R | TCGATCAGCTTCTTGCCCAGCC |
| Nox3ex-1F | TGTCATGCCGGTGTGCTGGA |
| Nox3ex-4R | CCCGTAGGCAACGAGTTTGTGGA |
| Nox3ex-9F | TCAGGAGACTGGACAGAGGCGT |
| Nox3ex-10F | ACCCCGTGAGCGTGTGCATT |
| Nox3ex-10R | AATGCACACGCTCACGGGGT |
| Nox3ex-11F | CTCACTGGCTGGGATGAAAACCA |
| Nox3ex-11R | CGGCATCCCGGCAGATCCAATA |
| Nox3ex-12R | TTCGTCGTTCCAGTTGGGTCGC |
| Nox3het–5J | |
| Amplification of duplication breakpoint | |
| Nox3-46393F | CCGAGGATCTCCATTACTGC |
| Nox3-44516R | GCAGATACCAAATTGCACTCTG |
| het-5JR | AAGACGTCTCCCATGTTCCA |
| Nox3R96 | |
| Genotyping | |
| Nox3R96-F | GCATTTGGCAGAAACCTCTT |
| Nox3R96-R | CACTGGGAGAGATGCACAAA |
| Nox3R542 | |
| Genotyping | |
| Nox3R542-F | AATAAAGCATTTCAAGCCGTG |
| Nox3R542-R | TTGTCTGGACTTCAGGGAGG |
The oligonucleotides shown here are those used for the genotyping, RACE, RT-PCR, and duplication breakpoint analyses of Nox3het, Nox3het–3J, Nox3het–4J, and Nox3het–5J alleles described in the text. Although the molecular lesions of the Nox3het–2J, Nox3R96, and Nox3R542 alleles have been described elsewhere, genotyping primers are provided here for the detection of those alleles. A HindIII site (in the 3′NoxInH oligonucleotide) and an XhoI site (in the 3′RaceInX oligonucleotide) that were used for cloning the 3′ RACE product from the Nox3het allele are underlined. Primer names and numbering are arbitrary