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. Author manuscript; available in PMC: 2012 Apr 1.
Published in final edited form as: Aging Cell. 2010 Dec 29;10(2):263–276. doi: 10.1111/j.1474-9726.2010.00660.x

Figure 6. Age-dependent changes in microglia aggregation and disaggregation following focal laser injury in vivo.

Figure 6

Four days following focal laser retinal injury in vivo, microglia in both young (A) and aged (B) animals were found to aggregate at an increased density at the site of the laser injury. At 16 days post-injury, microglia in young retina were more dispersed from the laser lesion site (C) while microglia in aged retina maintained their aggregation at the laser site (D). Quantification of microglial accumulation at the laser lesion site revealed that while microglia aggregation density 4 days post-laser was similar between young and aged retina, microglial disaggregation and dispersal occurred at 16 days post-laser in young animals but not as readily in aged retina. Asterisks * indicate significant (p<0.05) differences from baseline values, N.S. indicates comparisons for which p >0.05. Scale bar = 100 µm. The number of animals analyzed at the 4 day time point: young, n = 35 lesions from 9 animals, aged, n = 18 lesions from 5 animals. The number of animals analyzed at the 16 day time point: young, n = 32 lesions from 8 animals, aged, n = 20 lesions from 5 animals.