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. 2001 Jan;12(1):85–100. doi: 10.1091/mbc.12.1.85

Figure 1.

Figure 1

An antibody termed 2A3, against the G-domain of the α4 laminin subunit, was characterized by Western immunoblotting using SDS-PAGE preparations of whole cell extracts (WCE) (A) and matrix (ECM) (B) of TrHBMECs and HMVECs. In the Western blots of the whole cell extract and the matrix preparations, 2A3 antibody primarily reacts with a protein of an approximate molecular mass of 250 kDa. A polyclonal serum against the α4 laminin subunit recognizes a similar sized polypeptide in these preparations. A monoclonal antibody (RG13) against the α3 laminin subunit fails to recognize any polypeptides in either the extract or matrix of either cell type.