Figure 8.

TrHBMECs were processed for double-label immunogold localization using a mixture of mouse monoclonal antibodies against vimentin and a rabbit antiserum against β3-integrin. The binding of these probes was visualized with 6-nm gold-conjugated goat anti-mouse IgG and 18-nm gold-conjugated goat anti-rabbit IgG. Sections of the cells were prepared either parallel (A) or perpendicular (B and C) to their substratum-attached surface. In A, linear arrays of 6-nm gold particles arise from a cluster of 18-nm gold particles. A second cluster of 18-nm gold particles shows no association with the smaller sized gold particles in A. In B and C, strings of 6-nm particles interact with 18-nm gold clusters along the basal surface of the cells. Bar, 60 nm.